Study on Preparation of Immobilized Alkaline Protease and Its Performance

The nano-Fe3O4 particles were prepared by hydrothermal method. Cellulose-based magnetic Fe3O4 was prepared by adding cellulose solution dissolved in sodium hydroxide / thiourea / urea system. The basic protease was used as the catalyst and glutaraldehyde was used as the crosslinking agent, The production of magnetic recyclable immobilized enzyme can greatly reduce the cost of hydrolysis of sludge. The aldehyde groups in the glutaraldehyde and the amino groups in the protein can be stably crosslinked by coupling. The immobilization effect of nanometer Fe3O4 was reflected by investigating the activity of immobilized enzyme and immobilization rate of enzyme under different conditions.